Hotspot

rpl14-rpl16

LSC · 84673-84831 · 159 bp

Back to Atalantia

Pi 0.0674
MarkerSeek score 77.70
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
159
variable_sites
28
pi
0.0674
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.89
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 TCAAATGGGTTTGAGGTTGA CGTCCAAAATGCCTATAAGAACTC 40.0 41.7 55.0 58.0 205-228 bp 1.0 52.0
rpl14-rpl16_p2 TCAAATGGGTTTGAGGTTGA CGTCCAAAATGCCTATAAGAACT 40.0 39.1 55.0 56.9 205-228 bp 1.0 52.0
rpl14-rpl16_p3 TCAAATGGGTTTGAGGTTGAA CGTCCAAAATGCCTATAAGAACTC 38.1 41.7 55.9 58.0 205-228 bp 1.0 52.0
rpl14-rpl16_p4 TCAAATGGGTTTGAGGTTGAA CGTCCAAAATGCCTATAAGAACT 38.1 39.1 55.9 56.9 205-228 bp 1.0 52.0
rpl14-rpl16_p5 TGGGTTTGAGGTTGAATCAT CGTCCAAAATGCCTATAAGAACTC 40.0 41.7 54.8 58.0 200-223 bp 1.0 52.0