Hotspot

clpP1-clpP1

LSC · 73811-74468 · 658 bp

Back to Atalantia

Pi 0.017

MarkerSeek score 64.80

Primer available yes

Label clpP1-clpP1

Feature Metadata

label_name: clpP1-clpP1
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 29
pi: 0.017
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-clpP1_p1	TAAGGATTTCGCGCAGTCTC	TCCATGGGGTCTTTTCTCCT	50.0	50.0	58.1	57.9	814-834 bp	1.0	82.8
clpP1-clpP1_p2	ATTTCGCGCAGTCTCAGTAG	TCCATGGGGTCTTTTCTCCT	50.0	50.0	58.1	57.9	809-829 bp	1.0	82.8
clpP1-clpP1_p3	GATTTCGCGCAGTCTCAGTA	TCCATGGGGTCTTTTCTCCT	50.0	50.0	58.1	57.9	810-830 bp	1.0	82.7
clpP1-clpP1_p4	CCATTGTAAGGATTTCGCGC	TCCATGGGGTCTTTTCTCCT	50.0	50.0	57.9	57.9	820-840 bp	1.0	82.5
clpP1-clpP1_p5	TAAGGATTTCGCGCAGTCTC	AATTTGTGCGACCCGATGTA	50.0	45.0	58.1	57.9	861-881 bp	1.0	82.2