Hotspot

accD-psaI

LSC · 61601-62388 · 788 bp

Back to Atalantia

Pi 0.0215

MarkerSeek score 67.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 45
pi: 0.0215
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGCTGTTTTCCTTTGAA	TTGCCATTGCCGGAAATACT	45.0	45.0	57.1	58.2	811-888 bp	1.0	80.1
accD-psaI_p2	CACGGCTGTTTTCCTTTGAAT	TTGCCATTGCCGGAAATACT	42.9	45.0	57.6	58.2	811-888 bp	1.0	76.0
accD-psaI_p3	CACGGCTGTTTTCCTTTGAA	TGCCATTGCCGGAAATACTA	45.0	45.0	57.1	57.3	810-887 bp	1.0	75.6
accD-psaI_p4	CACGGCTGTTTTCCTTTGAA	CCGGAAATACTAGGCCCACT	45.0	55.0	57.1	58.9	802-879 bp	1.0	74.4
accD-psaI_p5	CACGGCTGTTTTCCTTTGAA	CATTGCCATTGCCGGAAATA	45.0	45.0	57.1	57.1	813-890 bp	1.0	74.2