Hotspot

trnT-GGU-psbD

LSC · 34053-35297 · 1245 bp

Back to Atalantia

Pi 0.0119

MarkerSeek score 65.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 43
pi: 0.0119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAAAACGAATCGGTCCCTCC	55.0	50.0	58.1	58.2	1367-1431 bp	1.0	83.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AAAAACGAATCGGTCCCTCC	50.0	50.0	58.1	58.2	1370-1434 bp	1.0	83.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAATCGGTCCCT	55.0	45.0	58.1	57.7	1369-1433 bp	1.0	82.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAATCGGTCCCT	50.0	45.0	58.1	57.7	1372-1436 bp	1.0	82.4
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	AAAAACGAATCGGTCCCTCC	50.0	50.0	58.3	58.2	1344-1408 bp	1.0	81.6