Hotspot

atpH-atpI

LSC · 14153-15443 · 1291 bp

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Pi 0.0118

MarkerSeek score 64.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1291
variable_sites: 41
pi: 0.0118
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1115-1378 bp	1.0	85.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1171-1434 bp	1.0	84.4
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1153-1416 bp	1.0	84.3
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1116-1379 bp	1.0	83.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.2	57.9	1175-1438 bp	1.0	83.8