Hotspot

trnS-GCU-trnG-UCC

LSC · 8384-9257 · 874 bp

Back to Atalantia

Pi 0.028
MarkerSeek score 70.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
874
variable_sites
57
pi
0.028
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCACTCAGCCATCTCTCC AACTGAAGGACCCCTTAACT 61.1 45.0 56.4 55.6 927-2105 bp 1.0 47.4
trnS-GCU-trnG-UCC_p2 CCACTCAGCCATCTCTCC AAACTGAAGGACCCCTTAACT 61.1 42.9 56.4 56.5 928-2106 bp 1.0 47.4
trnS-GCU-trnG-UCC_p3 CCACTCAGCCATCTCTCC AAACTGAAGGACCCCTTAAC 61.1 45.0 56.4 54.9 928-2106 bp 1.0 47.4
trnS-GCU-trnG-UCC_p4 CCACTCAGCCATCTCTCC ACTGAAGGACCCCTTAACTA 61.1 45.0 56.4 54.7 926-2104 bp 1.0 47.4
trnS-GCU-trnG-UCC_p5 CCACTCAGCCATCTCTCC TCAATTAAACTGAAGGACCCCT 61.1 40.9 56.4 57.0 934-2112 bp 1.0 47.3