Hotspot

rps16-trnQ-UUG

LSC · 5678-6998 · 1321 bp

Back to Atalantia

Pi 0.0169
MarkerSeek score 64.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1321
variable_sites
55
pi
0.0169
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 901-1427 bp 1.0 86.6
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 899-1425 bp 1.0 86.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 883-1409 bp 1.0 85.8
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 894-1420 bp 1.0 85.2
rps16-trnQ-UUG_p5 AGCGGATCATGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 900-1426 bp 1.0 84.2