Hotspot

trnK-UUU-rps16

LSC · 3936-4519 · 584 bp

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Pi 0.0179
MarkerSeek score 68.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
584
variable_sites
29
pi
0.0179
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCCGTACTTAAAAGCCGAG TATTTCACAGAAAGCGGGGG 50.0 50.0 58.0 57.9 649-700 bp 1.0 86.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TATTTCACAGAAAGCGGGGG 50.0 50.0 58.2 57.9 638-689 bp 1.0 85.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TATTTCACAGAAAGCGGGGG 50.0 50.0 58.2 57.9 637-688 bp 1.0 85.2
trnK-UUU-rps16_p4 AGCCGTACTTAAAAGCCGAG AAAGCGGGGGTTTTTACAGA 50.0 45.0 58.0 57.6 639-690 bp 1.0 84.5
trnK-UUU-rps16_p5 AGCCGTACTTAAAAGCCGAG AAGCGGGGGTTTTTACAGAA 50.0 45.0 58.0 57.6 638-689 bp 1.0 84.5