primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGCCGTACTTAAAAGCCGAG	TATTTCACAGAAAGCGGGGG	20	20	50.0	50.0	58.0	57.9	4.5	0.0	0.128754	3936	4519	649-700	1.000000	86.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TATTTCACAGAAAGCGGGGG	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.291203	3936	4519	638-689	1.000000	85.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	TATTTCACAGAAAGCGGGGG	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.291203	3936	4519	637-688	1.000000	85.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGCCGTACTTAAAAGCCGAG	AAAGCGGGGGTTTTTACAGA	20	20	50.0	45.0	58.0	57.6	4.5	0.0	0.374281	3936	4519	639-690	1.000000	84.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGCCGTACTTAAAAGCCGAG	AAGCGGGGGTTTTTACAGAA	20	20	50.0	45.0	58.0	57.6	4.5	0.0	0.374281	3936	4519	638-689	1.000000	84.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	11.1	16.9	0.104521	5678	6998	901-1427	1.000000	86.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCGGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	11.1	16.9	0.105939	5678	6998	899-1425	1.000000	86.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5678	6998	883-1409	1.000000	85.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5678	6998	894-1420	1.000000	85.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGCGGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	11.1	16.9	0.398456	5678	6998	900-1426	1.000000	84.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCACTCAGCCATCTCTCC	AACTGAAGGACCCCTTAACT	18	20	61.1	45.0	56.4	55.6	0.0	0.0	5.951915	8384	9257	927-2105	1.000000	47.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CCACTCAGCCATCTCTCC	AAACTGAAGGACCCCTTAACT	18	21	61.1	42.9	56.4	56.5	0.0	0.0	6.141991	8384	9257	928-2106	1.000000	47.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CCACTCAGCCATCTCTCC	AAACTGAAGGACCCCTTAAC	18	20	61.1	45.0	56.4	54.9	0.0	0.0	6.747285	8384	9257	928-2106	1.000000	47.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CCACTCAGCCATCTCTCC	ACTGAAGGACCCCTTAACTA	18	20	61.1	45.0	56.4	54.7	0.0	0.0	6.854479	8384	9257	926-2104	1.000000	47.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CCACTCAGCCATCTCTCC	TCAATTAAACTGAAGGACCCCT	18	22	61.1	40.9	56.4	57.0	0.0	0.0	6.611225	8384	9257	934-2112	1.000000	47.3
atpF-atpH_p1	atpF-atpH	1	TGGGTCCAGCCTATTCTTGA	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.072973	13464	13906	481-884	1.000000	91.4
atpF-atpH_p2	atpF-atpH	2	TGGGTCCAGCCTATTCTTGA	AAATCCTGTGTTGTGCCTGT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.106363	13464	13906	716-1109	1.000000	90.7
atpF-atpH_p3	atpF-atpH	3	TGGGTCCAGCCTATTCTTGA	TGGGCTTGCTTCTATTGGAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.163356	13464	13906	443-846	1.000000	90.7
atpF-atpH_p4	atpF-atpH	4	GTGCTGTGTCTGGACTTCAA	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	58.0	58.0	2.4	0.0	0.084828	13464	13906	692-1101	1.000000	90.5
atpF-atpH_p5	atpF-atpH	5	GTGCTGTGTCTGGACTTCAA	AAATCCTGTGTTGTGCCTGT	20	20	50.0	45.0	58.0	57.9	2.4	0.0	0.118219	13464	13906	927-1326	1.000000	89.5
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14153	15443	1115-1378	1.000000	85.1
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.245019	14153	15443	1171-1434	1.000000	84.4
atpH-atpI_p3	atpH-atpI	3	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.261511	14153	15443	1153-1416	1.000000	84.3
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	14153	15443	1116-1379	1.000000	83.9
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.323056	14153	15443	1175-1438	1.000000	83.8
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	20	20	35.0	50.0	52.0	58.5	0.0	5.4	6.474201	31224	32404	746-1266	1.000000	52.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ATGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	21	20	33.3	50.0	52.7	58.5	0.0	5.4	6.746812	31224	32404	747-1267	1.000000	52.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGCGAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	21	20	33.3	50.0	52.7	58.5	0.0	5.4	6.746812	31224	32404	746-1266	1.000000	52.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AATGCGAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	22	20	31.8	50.0	53.6	58.5	0.0	5.4	6.858000	31224	32404	748-1268	1.000000	52.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TGCGAGAATATTTACTTCCA	GTTCAATTGGTCAGAGCACC	20	20	35.0	50.0	52.0	57.0	0.0	5.4	6.994354	31224	32404	747-1267	1.000000	52.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	AAAAACGAATCGGTCCCTCC	20	20	55.0	50.0	58.1	58.2	7.6	7.8	0.246710	34053	35297	1367-1431	1.000000	83.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	AAAAACGAATCGGTCCCTCC	20	20	50.0	50.0	58.1	58.2	0.0	7.8	0.268865	34053	35297	1370-1434	1.000000	83.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAATCGGTCCCT	20	20	55.0	45.0	58.1	57.7	7.6	7.8	0.329656	34053	35297	1369-1433	1.000000	82.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAATCGGTCCCT	20	20	50.0	45.0	58.1	57.7	0.0	7.8	0.351811	34053	35297	1372-1436	1.000000	82.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	AAAAACGAATCGGTCCCTCC	20	20	50.0	50.0	58.3	58.2	0.0	7.8	0.465449	34053	35297	1344-1408	1.000000	81.6
accD-psaI_p1	accD-psaI	1	CACGGCTGTTTTCCTTTGAA	TTGCCATTGCCGGAAATACT	20	20	45.0	45.0	57.1	58.2	0.0	7.5	1.015456	61601	62388	811-888	1.000000	80.1
accD-psaI_p2	accD-psaI	2	CACGGCTGTTTTCCTTTGAAT	TTGCCATTGCCGGAAATACT	21	20	42.9	45.0	57.6	58.2	0.0	7.5	1.523540	61601	62388	811-888	1.000000	76.0
accD-psaI_p3	accD-psaI	3	CACGGCTGTTTTCCTTTGAA	TGCCATTGCCGGAAATACTA	20	20	45.0	45.0	57.1	57.3	0.0	7.5	1.580993	61601	62388	810-887	1.000000	75.6
accD-psaI_p4	accD-psaI	4	CACGGCTGTTTTCCTTTGAA	CCGGAAATACTAGGCCCACT	20	20	45.0	55.0	57.1	58.9	0.0	0.0	1.732405	61601	62388	802-879	1.000000	74.4
accD-psaI_p5	accD-psaI	5	CACGGCTGTTTTCCTTTGAA	CATTGCCATTGCCGGAAATA	20	20	45.0	45.0	57.1	57.1	0.0	7.5	1.748325	61601	62388	813-890	1.000000	74.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.194794	68042	69431	1443-1543	1.000000	77.1
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.194794	68042	69431	1444-1544	1.000000	77.0
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	20	20	50.0	45.0	57.8	56.9	24.9	0.0	1.299803	68042	69431	1367-1467	1.000000	76.4
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	20	20	50.0	45.0	57.8	56.9	24.9	0.0	1.299803	68042	69431	1366-1466	1.000000	76.4
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AGCCGCTAGTAGAAAACCAA	20	20	45.0	45.0	58.2	56.9	0.0	0.0	1.314480	68042	69431	1452-1552	1.000000	76.1
clpP1-clpP1_p1	clpP1-clpP1	1	TAAGGATTTCGCGCAGTCTC	TCCATGGGGTCTTTTCTCCT	20	20	50.0	50.0	58.1	57.9	8.7	13.0	0.137186	73811	74468	814-834	1.000000	82.8
clpP1-clpP1_p2	clpP1-clpP1	2	ATTTCGCGCAGTCTCAGTAG	TCCATGGGGTCTTTTCTCCT	20	20	50.0	50.0	58.1	57.9	8.7	13.0	0.145123	73811	74468	809-829	1.000000	82.8
clpP1-clpP1_p3	clpP1-clpP1	3	GATTTCGCGCAGTCTCAGTA	TCCATGGGGTCTTTTCTCCT	20	20	50.0	50.0	58.1	57.9	8.7	13.0	0.150381	73811	74468	810-830	1.000000	82.7
clpP1-clpP1_p4	clpP1-clpP1	4	CCATTGTAAGGATTTCGCGC	TCCATGGGGTCTTTTCTCCT	20	20	50.0	50.0	57.9	57.9	0.0	13.0	0.165079	73811	74468	820-840	1.000000	82.5
clpP1-clpP1_p5	clpP1-clpP1	5	TAAGGATTTCGCGCAGTCTC	AATTTGTGCGACCCGATGTA	20	20	50.0	45.0	58.1	57.9	8.7	0.0	0.179500	73811	74468	861-881	1.000000	82.2
psbT-pbf1_p1	psbT-pbf1	1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.6	58.4	0.0	0.0	0.787020	77935	77999	123-134	1.000000	83.2
psbT-pbf1_p2	psbT-pbf1	2	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.6	57.7	0.0	0.0	0.643202	77935	77999	171-182	1.000000	82.9
psbT-pbf1_p3	psbT-pbf1	3	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.5	58.4	0.0	0.0	0.895647	77935	77999	121-132	1.000000	82.4
psbT-pbf1_p4	psbT-pbf1	4	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.5	57.7	0.0	0.0	0.751829	77935	77999	169-180	1.000000	82.1
psbT-pbf1_p5	psbT-pbf1	5	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	20	20	50.0	50.0	57.6	57.4	0.0	0.0	0.920417	77935	77999	146-157	1.000000	81.3
rpl14_p1	rpl14	1	TCAGCAATAGTGTCCTTGCC	TGAGTCCTTTCATTCGCGAG	20	20	50.0	50.0	57.9	58.0	3.8	9.6	0.117569	84304	84672	1172-1212	1.000000	85.6
rpl14_p2	rpl14	2	TCACTATATTGCGGTCCCCT	TGAGTCCTTTCATTCGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	9.6	0.100818	84304	84672	1020-1043	1.000000	85.3
rpl14_p3	rpl14	3	TCCTATTCGAACCGTTCCCT	TGAGTCCTTTCATTCGCGAG	20	20	50.0	50.0	58.1	58.0	0.0	9.6	0.147079	84304	84672	1230-1270	0.875000	79.6
rpl14_p4	rpl14	4	AAAAGAACCCGGAGCATACC	TGAGTCCTTTCATTCGCGAG	20	20	50.0	50.0	57.9	58.0	0.7	9.6	0.127986	84304	84672	1679-1716	0.875000	78.4
rpl14_p5	rpl14	5	TCGTCGATGTCGTAGGGTTA	TGAGTCCTTTCATTCGCGAG	20	20	50.0	50.0	58.0	58.0	4.2	9.6	0.026473	84304	84672	1366-1390	0.375000	56.0
rpl14-rpl16_p1	rpl14-rpl16	1	TCAAATGGGTTTGAGGTTGA	CGTCCAAAATGCCTATAAGAACTC	20	24	40.0	41.7	55.0	58.0	0.0	0.0	6.999595	84673	84831	205-228	1.000000	52.0
rpl14-rpl16_p2	rpl14-rpl16	2	TCAAATGGGTTTGAGGTTGA	CGTCCAAAATGCCTATAAGAACT	20	23	40.0	39.1	55.0	56.9	0.0	0.0	7.021486	84673	84831	205-228	1.000000	52.0
rpl14-rpl16_p3	rpl14-rpl16	3	TCAAATGGGTTTGAGGTTGAA	CGTCCAAAATGCCTATAAGAACTC	21	24	38.1	41.7	55.9	58.0	0.0	0.0	7.171796	84673	84831	205-228	1.000000	52.0
rpl14-rpl16_p4	rpl14-rpl16	4	TCAAATGGGTTTGAGGTTGAA	CGTCCAAAATGCCTATAAGAACT	21	23	38.1	39.1	55.9	56.9	0.0	0.0	7.193687	84673	84831	205-228	1.000000	52.0
rpl14-rpl16_p5	rpl14-rpl16	5	TGGGTTTGAGGTTGAATCAT	CGTCCAAAATGCCTATAAGAACTC	20	24	40.0	41.7	54.8	58.0	0.0	0.0	7.240687	84673	84831	200-223	1.000000	52.0
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACTAAAGGTATGATCCAGGA	TCCAAAGATTTTTACGAATACG	23	22	43.5	31.8	57.1	52.9	0.0	0.0	10.950336	116620	117651	1116-1177	1.000000	48.5
ndhF-rpl32_p2	ndhF-rpl32	2	GGAACTAAAGGTATGATCCAGGA	TTCCAAAGATTTTTACGAATACG	23	23	43.5	30.4	57.1	53.8	0.0	0.0	11.128394	116620	117651	1117-1178	1.000000	48.5
ndhF-rpl32_p3	ndhF-rpl32	3	GGAACTAAAGGTATGATCCAGGAA	TCCAAAGATTTTTACGAATACG	24	22	41.7	31.8	57.8	52.9	0.0	0.0	11.309923	116620	117651	1116-1177	1.000000	48.5
ndhF-rpl32_p4	ndhF-rpl32	4	GGAACTAAAGGTATGATCCAGGA	TTTCCAAAGATTTTTACGAATACG	23	24	43.5	29.2	57.1	54.5	0.0	0.0	11.373245	116620	117651	1118-1179	1.000000	48.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGAACTAAAGGTATGATCCAGG	TCCAAAGATTTTTACGAATACG	22	22	45.5	31.8	55.7	52.9	0.0	0.0	11.389876	116620	117651	1116-1177	1.000000	48.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGCGAAATCCCTTGCTACTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037669	117811	118539	746-841	1.000000	88.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGCGAAATCCCTTGCTACTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039039	117811	118539	747-842	1.000000	88.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGCGAAATCCCTTGCTACTG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.154115	117811	118539	756-851	1.000000	87.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGCGAAATCCCTTGCTACTG	GCTGCATAGCCCAATACCAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.114697	117811	118539	1351-1440	1.000000	86.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGCGAAATCCCTTGCTACTG	CTTGGTGCCGTTATATCGCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.163973	117811	118539	1219-1293	0.375000	58.3