Hotspot

rpl22

LSC · 83944-84342 · 399 bp

Back to Astrocaryum

Pi 0.00227

MarkerSeek score 31.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 6
pi: 0.00227
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	AAGGAACGATGACTTTGGGT	TCATTAGTGGGGGATGACCT	45.0	50.0	57.0	57.4	531-534 bp	0.941176	66.8
rpl22_p2	AGGAACGATGACTTTGGGTT	TCATTAGTGGGGGATGACCT	45.0	50.0	57.0	57.4	530-533 bp	0.941176	66.8
rpl22_p3	GGAACGATGACTTTGGGTTG	TCATTAGTGGGGGATGACCT	50.0	50.0	57.0	57.4	529-532 bp	0.941176	66.5
rpl22_p4	CCAAGTCTGAAACCAAGTGGA	TCATTAGTGGGGGATGACCT	47.6	50.0	58.1	57.4	507-510 bp	0.941176	66.0
rpl22_p5	AAGGAACGATGACTTTGGGTT	TCATTAGTGGGGGATGACCT	42.9	50.0	57.8	57.4	531-534 bp	0.941176	64.7