Hotspot

rps15-ycf1

SSC · 124276-124667 · 392 bp

Back to Astrocaryum

Pi 0.00705

MarkerSeek score 40.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 392
variable_sites: 10
pi: 0.00705
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGAACCCTTGTTTTCT	TGGCAGTCGTTTCAGTATGT	38.1	45.0	55.5	57.2	518-519 bp	1.0	47.2
rps15-ycf1_p2	TCAACAGAACCCTTGTTTTCTTC	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.4	57.2	518-519 bp	1.0	46.3
rps15-ycf1_p3	CAACAGAACCCTTGTTTTCTTCT	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.4	57.2	517-518 bp	1.0	46.2
rps15-ycf1_p4	TGAAATTCAACAGAACCCTTGT	TGGCAGTCGTTTCAGTATGT	36.4	45.0	56.4	57.2	524-525 bp	1.0	45.9
rps15-ycf1_p5	TCAACAGAACCCTTGTTTTCT	GCAGTCGTTTCAGTATGTCG	38.1	50.0	55.5	57.0	516-517 bp	1.0	45.5