Hotspot

psaC-ndhE

SSC · 117938-118566 · 629 bp

Back to Astrocaryum

Pi 0.00497
MarkerSeek score 57.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
629
variable_sites
20
pi
0.00497
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA CTGAAGCAGCTATTGGGCTA 50.0 50.0 58.1 57.4 823-865 bp 1.0 79.9
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGCTAG 50.0 50.0 58.1 57.4 822-864 bp 1.0 79.9
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA TGTTATAGCTGTTGCGGCTG 50.0 50.0 58.1 58.6 843-885 bp 1.0 79.9
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA GAAGCAGCTATTGGGCTAGC 50.0 55.0 58.1 58.8 821-863 bp 1.0 78.8
psaC-ndhE_p5 CAATACATCTGTGGGGCAGG TGAAGCAGCTATTGGGCTAG 55.0 50.0 58.3 57.4 798-840 bp 1.0 78.5