Hotspot

petN-psbM

LSC · 29390-29903 · 514 bp

Back to Astrocaryum

Pi 0.0063

MarkerSeek score 61.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 16
pi: 0.0063
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	670-691 bp	1.0	72.3
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	615-636 bp	1.0	71.4
petN-psbM_p3	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.7	57.6	665-686 bp	1.0	71.3
petN-psbM_p4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.6	57.6	674-695 bp	1.0	69.3
petN-psbM_p5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.6	57.6	673-694 bp	1.0	68.6