Hotspot

rpoB-trnC-GCA

LSC · 27256-28471 · 1216 bp

Back to Astrocaryum

Pi 0.00133

MarkerSeek score 52.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 8
pi: 0.00133
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1340-1376 bp	1.0	76.7
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1260-1296 bp	1.0	75.0
rpoB-trnC-GCA_p3	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1261-1297 bp	1.0	74.8
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1377-1413 bp	1.0	74.7
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	GACACCCGGATTTGAACTGG	50.0	55.0	58.2	58.8	1363-1399 bp	1.0	74.2