Hotspot

ndhH

SSC · 113604-114785 · 1182 bp

Back to Astrebla

Pi 0.00338

MarkerSeek score 60.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 4
pi: 0.00338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCAGTAGGAGAGTCAAAA	TGCATTTTGATGAGCCTCGT	45.0	45.0	55.1	58.2	1361 bp	1.0	55.5
ndhH_p2	CCCAGTAGGAGAGTCAAAATAGG	TGCATTTTGATGAGCCTCGT	47.8	45.0	57.7	58.2	1360 bp	1.0	52.6
ndhH_p3	TCCCAGTAGGAGAGTCAAAAT	TGCATTTTGATGAGCCTCGT	42.9	45.0	55.7	58.2	1361 bp	1.0	52.3
ndhH_p4	TCCCAGTAGGAGAGTCAAAA	TTGCATTTTGATGAGCCTCG	45.0	45.0	55.1	57.1	1362 bp	1.0	49.5
ndhH_p5	TCCCAGTAGGAGAGTCAAAA	GCATTTTGATGAGCCTCGTT	45.0	45.0	55.1	57.1	1360 bp	1.0	49.5