Hotspot

rbcL-psaI

LSC · 57022-57909 · 888 bp

Back to Astrebla

Pi 0.00788

MarkerSeek score 63.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 888
variable_sites: 7
pi: 0.00788
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	982 bp	1.0	80.3
rbcL-psaI_p2	TCACATTCGACTACAAGCCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	996 bp	1.0	80.3
rbcL-psaI_p3	TCGACTACAAGCCGGTAGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	990 bp	1.0	80.0
rbcL-psaI_p4	CAAGCCGGTAGATACCATCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	983 bp	1.0	79.8
rbcL-psaI_p5	AAGCCGGTAGATACCATCGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	988 bp	1.0	79.7