Hotspot

petN-trnC-GCA

LSC · 18217-19063 · 847 bp

Back to Astrebla

Pi 0.00118

MarkerSeek score 59.40

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 1
pi: 0.00118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTTCTCCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	955 bp	1.0	70.7
petN-trnC-GCA_p2	GTCCACTTCTCCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	954 bp	1.0	70.7
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	896 bp	1.0	70.5
petN-trnC-GCA_p4	AGTCCACTTCTCCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	957 bp	1.0	70.3
petN-trnC-GCA_p5	GTCCACTTCTCCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	956 bp	1.0	70.3