Hotspot

trnD-GUC-psbM

LSC · 16141-17226 · 1086 bp

Back to Astrebla

Pi 0.00184
MarkerSeek score 59.80
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1086
variable_sites
2
pi
0.00184
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TCAATTGGTTAGAGCACCGC TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.6 56.7 1250-1253 bp 1.0 65.3
trnD-GUC-psbM_p2 TAGGTATGCCATACACCCCG TCATTTTGGCTGGCTGTTTT 55.0 40.0 58.7 56.7 1283-1286 bp 1.0 64.5
trnD-GUC-psbM_p3 TCAATTGGTTAGAGCACCGC AATCATTTTGGCTGGCTGTT 50.0 40.0 58.6 56.5 1252-1255 bp 1.0 63.5
trnD-GUC-psbM_p4 TCAATTGGTTAGAGCACCGC ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.6 56.5 1251-1254 bp 1.0 63.5
trnD-GUC-psbM_p5 TCAATTGGTTAGAGCACCGC TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.6 57.4 1250-1253 bp 1.0 63.3