Hotspot

rps16-trnQ-UUG

LSC · 5679-6990 · 1312 bp

Back to Astrebla

Pi 0.000762

MarkerSeek score 59.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1312
variable_sites: 1
pi: 0.000762
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1385-1390 bp	1.0	78.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CGCATAAAAGAAATGGGGCG	45.0	50.0	58.1	58.2	1446-1451 bp	1.0	77.7
rps16-trnQ-UUG_p3	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1396-1401 bp	1.0	76.5
rps16-trnQ-UUG_p4	TCATCTCCTTCAAGTCGCAC	CGCATAAAAGAAATGGGGCG	50.0	50.0	57.6	58.2	1457-1462 bp	1.0	75.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1389-1394 bp	1.0	73.6