primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5679	6990	1385-1390	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	CGCATAAAAGAAATGGGGCG	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.300411	5679	6990	1446-1451	1.000000	77.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.446382	5679	6990	1396-1401	1.000000	76.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATCTCCTTCAAGTCGCAC	CGCATAAAAGAAATGGGGCG	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.531368	5679	6990	1457-1462	1.000000	75.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5679	6990	1389-1394	1.000000	73.6
trnG-UCC_p1	trnG-UCC	1	GCTCTACTCCGCTCTGTAGA	CTCGAATGGCTACCCTTGAC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.102378	13144	13896	909-909	1.000000	79.3
trnG-UCC_p2	trnG-UCC	2	GCTCTACTCCGCTCTGTAGA	CGAATGGCTACCCTTGACAA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.151653	13144	13896	907-907	1.000000	78.9
trnG-UCC_p3	trnG-UCC	3	GCTCTACTCCGCTCTGTAGA	GAAGTACCAACTGCTCGCTT	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.246679	13144	13896	956-956	1.000000	78.1
trnG-UCC_p4	trnG-UCC	4	GCTCTACTCCGCTCTGTAGA	AAGTACCAACTGCTCGCTTT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.299820	13144	13896	955-955	1.000000	77.7
trnG-UCC_p5	trnG-UCC	5	GCTCTACTCCGCTCTGTAGA	AGTACCAACTGCTCGCTTTT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.299820	13144	13896	954-954	1.000000	77.7
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTT	20	20	50.0	40.0	58.6	56.7	5.4	2.0	1.848735	16141	17226	1250-1253	1.000000	65.3
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	20	20	55.0	40.0	58.7	56.7	16.3	2.0	1.953655	16141	17226	1283-1286	1.000000	64.5
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TCAATTGGTTAGAGCACCGC	AATCATTTTGGCTGGCTGTT	20	20	50.0	40.0	58.6	56.5	5.4	2.0	2.080552	16141	17226	1252-1255	1.000000	63.5
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TCAATTGGTTAGAGCACCGC	ATCATTTTGGCTGGCTGTTT	20	20	50.0	40.0	58.6	56.5	5.4	2.0	2.080552	16141	17226	1251-1254	1.000000	63.5
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTTT	20	21	50.0	38.1	58.6	57.4	5.4	2.0	2.108244	16141	17226	1250-1253	1.000000	63.3
psbM-petN_p1	psbM-petN	1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	20	20	40.0	55.0	56.7	58.1	0.0	0.0	1.416779	17332	18126	843-844	1.000000	69.0
psbM-petN_p2	psbM-petN	2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	20	20	40.0	55.0	56.7	58.1	0.0	0.0	1.416779	17332	18126	842-843	1.000000	69.0
psbM-petN_p3	psbM-petN	3	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	20	20	40.0	50.0	56.7	58.2	0.0	0.0	1.449068	17332	18126	901-902	1.000000	68.8
psbM-petN_p4	psbM-petN	4	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	20	20	40.0	55.0	56.7	57.8	0.0	0.0	1.473967	17332	18126	841-842	1.000000	68.6
psbM-petN_p5	psbM-petN	5	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	20	20	40.0	60.0	56.7	58.2	0.0	0.0	1.514649	17332	18126	838-839	1.000000	68.3
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGTCCACTTCTCCCCCATAC	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	18217	19063	955-955	1.000000	70.7
petN-trnC-GCA_p2	petN-trnC-GCA	2	GTCCACTTCTCCCCCATACT	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	18217	19063	954-954	1.000000	70.7
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	18217	19063	896-896	1.000000	70.5
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGTCCACTTCTCCCCCATAC	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	18217	19063	957-957	1.000000	70.3
petN-trnC-GCA_p5	petN-trnC-GCA	5	GTCCACTTCTCCCCCATACT	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	18217	19063	956-956	1.000000	70.3
atpI_p1	atpI	1	GCTGAGTTGAGAAAGAGATGGT	TGCATGAATTGGGCTAAGCT	22	20	45.5	45.0	58.1	57.9	0.0	0.0	2.211882	31460	32203	869-869	1.000000	62.4
atpI_p2	atpI	2	TGCTGAGTTGAGAAAGAGATGG	TGCATGAATTGGGCTAAGCT	22	20	45.5	45.0	58.1	57.9	0.0	0.0	2.213130	31460	32203	870-870	1.000000	62.4
atpI_p3	atpI	3	ACTTCAAGTTGCTGAGTTGAGA	TGCATGAATTGGGCTAAGCT	22	20	40.9	45.0	57.9	57.9	0.0	0.0	2.291054	31460	32203	879-879	1.000000	61.8
atpI_p4	atpI	4	GCTGAGTTGAGAAAGAGATGGT	CAACCATGCATGAATTGGGC	22	20	45.5	50.0	58.1	58.3	0.0	28.3	2.404442	31460	32203	875-875	1.000000	60.9
atpI_p5	atpI	5	TGCTGAGTTGAGAAAGAGATGG	CAACCATGCATGAATTGGGC	22	20	45.5	50.0	58.1	58.3	0.0	28.3	2.405691	31460	32203	876-876	1.000000	60.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	46827	47662	937-937	1.000000	79.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	46827	47662	929-929	1.000000	79.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	46827	47662	907-907	1.000000	79.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	46827	47662	927-927	1.000000	77.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	46827	47662	936-936	1.000000	77.7
rbcL-psaI_p1	rbcL-psaI	1	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	1.9	26.9	0.035882	57022	57909	982-982	1.000000	80.3
rbcL-psaI_p2	rbcL-psaI	2	TCACATTCGACTACAAGCCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.036204	57022	57909	996-996	1.000000	80.3
rbcL-psaI_p3	rbcL-psaI	3	TCGACTACAAGCCGGTAGAT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	16.7	26.9	0.075447	57022	57909	990-990	1.000000	80.0
rbcL-psaI_p4	rbcL-psaI	4	CAAGCCGGTAGATACCATCG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	57.9	58.0	1.9	26.9	0.092996	57022	57909	983-983	1.000000	79.8
rbcL-psaI_p5	rbcL-psaI	5	AAGCCGGTAGATACCATCGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	1.9	26.8	0.112416	57022	57909	988-988	1.000000	79.7
petA_p1	petA	1	AATTCCGGGATCCACGATTG	TTATTGTTCTCGCCTCTGCC	20	20	50.0	50.0	58.0	58.0	20.2	0.0	0.056857	60216	61178	1094-1094	1.000000	79.8
petA_p2	petA	2	GAAATTCCGGGATCCACGAT	TTATTGTTCTCGCCTCTGCC	20	20	50.0	50.0	57.7	58.0	20.2	0.0	0.280293	60216	61178	1096-1096	1.000000	78.0
petA_p3	petA	3	AATTCCGGGATCCACGATTG	TCTGCCTTGGATTCTCCTCT	20	20	50.0	50.0	58.0	57.7	20.2	0.0	0.293320	60216	61178	1080-1080	1.000000	77.9
petA_p4	petA	4	GAAATTCCGGGATCCACGAT	TCTGCCTTGGATTCTCCTCT	20	20	50.0	50.0	57.7	57.7	20.2	0.0	0.516756	60216	61178	1082-1082	1.000000	76.2
petA_p5	petA	5	AATTCCGGGATCCACGATTG	CTCTGCCTTGGATTCTCCTC	20	20	50.0	55.0	58.0	57.4	20.2	0.0	0.651123	60216	61178	1081-1081	1.000000	75.1
petA-psbJ_p1	petA-psbJ	1	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	20	21	50.0	47.6	58.7	58.1	17.6	0.0	1.780175	61179	62048	1014-1014	1.000000	65.8
petA-psbJ_p2	petA-psbJ	2	GATCCATTACGCGTCCAAGG	ACGGTTCGTATTCTGGATTGG	20	21	55.0	47.6	58.8	58.1	18.6	0.0	1.880870	61179	62048	1025-1025	1.000000	65.0
petA-psbJ_p3	petA-psbJ	3	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	20	21	50.0	47.6	58.7	57.7	17.6	0.0	1.958565	61179	62048	1012-1012	1.000000	64.4
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	20	21	50.0	52.4	58.7	57.6	17.6	0.0	2.047863	61179	62048	1070-1070	1.000000	63.7
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	20	21	50.0	52.4	58.7	57.6	17.6	0.0	2.047863	61179	62048	1071-1071	1.000000	63.7
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	62818	64094	1435-1435	1.000000	76.2
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	62818	64094	1433-1433	1.000000	76.1
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	62818	64094	1424-1424	1.000000	75.9
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	62818	64094	1422-1422	1.000000	75.9
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	62818	64094	1426-1426	1.000000	74.4
rpl16_p1	rpl16	1	CTCCAGAAGGAAAACCTGGG	AGAGCCTTACCAAAAATCCCA	20	21	55.0	42.9	57.8	57.2	0.0	0.0	2.032484	78271	79752	1629-1630	1.000000	64.1
rpl16_p2	rpl16	2	CTCCAGAAGGAAAACCTGGG	AGCCTTACCAAAAATCCCAGA	20	21	55.0	42.9	57.8	57.2	0.0	0.0	2.032484	78271	79752	1627-1628	1.000000	64.1
rpl16_p3	rpl16	3	CTCCAGAAGGAAAACCTGGG	AAGAGCCTTACCAAAAATCCCA	20	22	55.0	40.9	57.8	57.9	0.0	0.0	2.333096	78271	79752	1630-1631	1.000000	61.7
rpl16_p4	rpl16	4	CTCCAGAAGGAAAACCTGGG	AGCCTTACCAAAAATCCCAGAA	20	22	55.0	40.9	57.8	57.9	0.0	0.0	2.333096	78271	79752	1627-1628	1.000000	61.7
rpl16_p5	rpl16	5	CTCCAGAAGGAAAACCTGGG	GAGCCTTACCAAAAATCCCAG	20	21	55.0	47.6	57.8	56.8	0.0	0.0	2.357908	78271	79752	1628-1629	1.000000	61.5
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	TAAGGAGGGGGATGCCTAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.9	57.8	2.2	0.0	0.285225	93916	94223	484-484	1.000000	78.2
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	TAAGGAGGGGGATGCCTAAG	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	57.9	57.8	2.2	4.7	0.288066	93916	94223	503-503	1.000000	78.2
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	TAAGGAGGGGGATGCCTAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.9	58.2	2.2	0.0	0.308206	93916	94223	452-452	1.000000	78.1
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	GTAAGGAGGGGGATGCCTAA	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	58.2	57.8	2.2	0.0	0.351064	93916	94223	485-485	1.000000	77.7
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	GTAAGGAGGGGGATGCCTAA	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	58.2	57.8	2.2	4.7	0.353906	93916	94223	504-504	1.000000	77.6
ndhF-rpl32_p1	ndhF-rpl32	1	TAAGAAAAAGCCCCAACCCC	TGACATCGAAGTACGTTTTT	20	20	50.0	35.0	58.0	53.2	0.0	0.0	4.785110	104328	105240	1031-1031	1.000000	41.8
ndhF-rpl32_p2	ndhF-rpl32	2	TAAGAAAAAGCCCCAACCCC	TTGACATCGAAGTACGTTTT	20	20	50.0	35.0	58.0	53.2	0.0	0.0	4.785110	104328	105240	1032-1032	1.000000	41.8
ndhF-rpl32_p3	ndhF-rpl32	3	TAAGAAAAAGCCCCAACCCC	TTTGACATCGAAGTACGTTT	20	20	50.0	35.0	58.0	53.2	0.0	0.0	4.785110	104328	105240	1033-1033	1.000000	41.8
ndhF-rpl32_p4	ndhF-rpl32	4	TAAGAAAAAGCCCCAACCCC	TTTTGACATCGAAGTACGTT	20	20	50.0	35.0	58.0	53.2	0.0	0.0	4.785110	104328	105240	1034-1034	1.000000	41.8
ndhF-rpl32_p5	ndhF-rpl32	5	TAAGAAAAAGCCCCAACCCC	TTTTTGACATCGAAGTACGT	20	20	50.0	35.0	58.0	53.2	0.0	0.0	4.785110	104328	105240	1035-1035	1.000000	41.8
ndhH_p1	ndhH	1	TCCCAGTAGGAGAGTCAAAA	TGCATTTTGATGAGCCTCGT	20	20	45.0	45.0	55.1	58.2	0.0	0.0	3.093462	113604	114785	1361-1361	1.000000	55.5
ndhH_p2	ndhH	2	CCCAGTAGGAGAGTCAAAATAGG	TGCATTTTGATGAGCCTCGT	23	20	47.8	45.0	57.7	58.2	0.0	0.0	3.458367	113604	114785	1360-1360	1.000000	52.6
ndhH_p3	ndhH	3	TCCCAGTAGGAGAGTCAAAAT	TGCATTTTGATGAGCCTCGT	21	20	42.9	45.0	55.7	58.2	0.0	0.0	3.488750	113604	114785	1361-1361	1.000000	52.3
ndhH_p4	ndhH	4	TCCCAGTAGGAGAGTCAAAA	TTGCATTTTGATGAGCCTCG	20	20	45.0	45.0	55.1	57.1	0.0	0.0	3.844131	113604	114785	1362-1362	1.000000	49.5
ndhH_p5	ndhH	5	TCCCAGTAGGAGAGTCAAAA	GCATTTTGATGAGCCTCGTT	20	20	45.0	45.0	55.1	57.1	0.0	0.0	3.846106	113604	114785	1360-1360	1.000000	49.5
trnI-GAU_p1	trnI-GAU	1	AGTGCATCAAGCTATTCGCA	AACTCCAAAAACCCGTCCTC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.051364	121901	122688	1773-1773	1.000000	79.7
trnI-GAU_p2	trnI-GAU	2	GCCAAGTGGAGTATGCATGA	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	10.2	0.0	0.065630	121901	122688	1422-1422	1.000000	79.6
trnI-GAU_p3	trnI-GAU	3	TCTGACCAACTTCCCATCCT	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067736	121901	122688	1657-1657	1.000000	79.6
trnI-GAU_p4	trnI-GAU	4	AGCCAAGTGGAGTATGCATG	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	10.2	0.0	0.071388	121901	122688	1423-1423	1.000000	79.6
trnI-GAU_p5	trnI-GAU	5	AGTGCATCAAGCTATTCGCA	CTGGCCAGGTTTGAAGTCAT	20	20	45.0	50.0	58.0	58.1	0.0	26.1	0.113279	121901	122688	1631-1631	1.000000	79.2