Hotspot

ycf1

Genome · 104080-109275 · 5196 bp

Back to Astragalus

Pi 0.0712

MarkerSeek score 76.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5196
variable_sites: 3031
pi: 0.0712
unique_haplotype_count: 62
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGATGCTTTCGTGGACAAA	AAATCCGGTTCGCGTGAATA	45.0	45.0	58.0	58.0	80-909 bp	0.590909	75.3
ycf1_p2	TGGATGCTTTCGTGGACAAA	TCCGGTTCGCGTGAATAATT	45.0	45.0	58.0	58.0	80-906 bp	0.530303	72.9
ycf1_p3	TGGATGCTTTCGTGGACAAA	AATCCGGTTCGCGTGAATAA	45.0	45.0	58.0	58.0	80-908 bp	0.515152	72.3
ycf1_p4	AACTGACGGAAGGATCCTCA	AATCCGGTTCGCGTGAATAA	50.0	45.0	58.1	58.0	80-213 bp	0.121212	56.3
ycf1_p5	AACTGACGGAAGGATCCTCA	TCCGGTTCGCGTGAATAATT	50.0	45.0	58.1	58.0	80-211 bp	0.121212	56.3