Hotspot

accD

Genome · 55134-56636 · 1503 bp

Back to Astragalus

Pi 0.0856

MarkerSeek score 75.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 1503
variable_sites: 965
pi: 0.0856
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATGGGTGGTATGTTGCTGTC	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.9	57.9	80-2932 bp	0.757576	80.7
accD_p2	GGGTGGTATGTTGCTGTCAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.9	57.9	80-2930 bp	0.757576	80.7
accD_p3	GGGTGGTATGTTGCTGTCAT	AGGCACAAAAACGGAAGGTA	50.0	45.0	57.9	57.6	80-2895 bp	0.742424	77.9
accD_p4	AGGGGAGGGATTGATCATGT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.8	57.9	80-2969 bp	0.393939	65.4
accD_p5	GGGATAGGGGAGGGATTGAT	GTCATTGCAATTGCCGGAAA	55.0	45.0	57.6	57.9	80-2974 bp	0.409091	64.8