Hotspot

rpl32-ndhF

Genome · 120042-120785 · 744 bp

Back to Astragalus

Pi 0.059

MarkerSeek score 81.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 744
variable_sites: 505
pi: 0.059
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AAGGCTTTCAATGCTGTCCA	AGGGCGCAGAAAGTAACAAA	45.0	45.0	58.0	58.0	80-1592 bp	0.818182	84.5
rpl32-ndhF_p2	AGGCTTTCAATGCTGTCCAA	AGGGCGCAGAAAGTAACAAA	45.0	45.0	58.0	58.0	80-1591 bp	0.818182	84.5
rpl32-ndhF_p3	AGGCTTTCAATGCTGTCCAA	TTTTTGACAAGCCTTTGCCG	45.0	45.0	58.0	58.1	80-1430 bp	0.818182	84.2
rpl32-ndhF_p4	AAGGCTTTCAATGCTGTCCA	TGTTGCCGTGGGAAAAAGTA	45.0	45.0	58.0	57.9	80-999 bp	0.727273	80.7
rpl32-ndhF_p5	AGGCTTTCAATGCTGTCCAA	TGTTGCCGTGGGAAAAAGTA	45.0	45.0	58.0	57.9	80-998 bp	0.727273	80.7