Hotspot

trnP-UGG-psaJ

Genome · 63887-64444 · 558 bp

Back to Astragalus

Pi 0.0596

MarkerSeek score 85.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 558
variable_sites: 322
pi: 0.0596
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	ACTGGTGCCACAGAGAGATA	45.0	50.0	57.9	57.8	80-722 bp	0.939394	86.8
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	ACTGGTGCCACAGAGAGATA	45.0	50.0	58.2	57.8	80-723 bp	0.939394	86.3
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	ACTGGTGCCACAGAGAGATA	45.0	50.0	57.8	57.8	80-730 bp	0.939394	86.1
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	ACCGCGAACCATAGAGTACT	45.0	50.0	57.9	58.2	80-746 bp	0.924242	86.1
trnP-UGG-psaJ_p5	CAAAACAAACGCGCTACCAA	ACCGCGAACCATAGAGTACT	45.0	50.0	58.2	58.2	80-747 bp	0.924242	85.7