Hotspot

petA-psbJ

Genome · 60229-61114 · 886 bp

Back to Astragalus

Pi 0.0499

MarkerSeek score 82.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 886
variable_sites: 460
pi: 0.0499
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	TGGTTCGTATTCCGGATTGG	42.9	50.0	57.2	57.7	80-988 bp	0.939394	72.9
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	GGTTCGTATTCCGGATTGGG	42.9	55.0	57.2	58.4	80-987 bp	0.939394	72.2
petA-psbJ_p3	GCGTCTATTATTTTGGCCCA	TGGTTCGTATTCCGGATTGG	45.0	50.0	56.2	57.7	80-1038 bp	0.924242	71.8
petA-psbJ_p4	GCGTCTATTATTTTGGCCCA	GGTTCGTATTCCGGATTGGG	45.0	55.0	56.2	58.4	80-1037 bp	0.924242	71.1
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGA	GTTCGTATTCCGGATTGGGA	42.9	50.0	57.2	57.4	80-986 bp	0.939394	70.6