Hotspot

trnG-UCC-trnS-GCU

Genome · 51810-52457 · 648 bp

Back to Astragalus

Pi 0.0612
MarkerSeek score 80.90
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
Genome
length_bp
648
variable_sites
363
pi
0.0612
unique_haplotype_count
58
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GTCCTTAGAACGAATCGCACT CAATCCGACGCTTTAGTCCA 47.6 50.0 58.1 58.0 520-865 bp 0.969697 81.8
trnG-UCC-trnS-GCU_p2 GTCCTTAGAACGAATCGCACT AATCCGACGCTTTAGTCCAC 47.6 50.0 58.1 58.0 519-864 bp 0.969697 81.8
trnG-UCC-trnS-GCU_p3 ACGAATCGCACTTTTACCACT CAATCCGACGCTTTAGTCCA 42.9 50.0 58.2 58.0 511-856 bp 0.969697 80.9
trnG-UCC-trnS-GCU_p4 ACGAATCGCACTTTTACCACT AATCCGACGCTTTAGTCCAC 42.9 50.0 58.2 58.0 510-855 bp 0.969697 80.8
trnG-UCC-trnS-GCU_p5 ACGAATCGCACTTTTACCAC CAATCCGACGCTTTAGTCCA 45.0 50.0 56.8 58.0 511-856 bp 0.969697 80.8