Hotspot

trnC-GCA-rpoB

Genome · 32808-33958 · 1151 bp

Back to Astragalus

Pi 0.0531

MarkerSeek score 87.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: Genome
length_bp: 1151
variable_sites: 580
pi: 0.0531
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CTTACCACTCGGCCATATCG	TTCATTTCCAACCCCAAGCA	55.0	45.0	57.9	57.9	1041-1273 bp	0.939394	88.3
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	TTCATTTCCAACCCCAAGCA	50.0	45.0	58.1	57.9	1081-1313 bp	0.909091	87.0
trnC-GCA-rpoB_p3	CTTACCACTCGGCCATATCG	TCATTTCCAACCCCAAGCAT	55.0	45.0	57.9	57.7	1040-1272 bp	0.939394	86.5
trnC-GCA-rpoB_p4	ATTCTCTTGTCGATCAGGCG	TTCATTTCCAACCCCAAGCA	50.0	45.0	57.8	57.9	1105-1337 bp	0.893939	85.3
trnC-GCA-rpoB_p5	CCGGATTTGAACTGGGGAAA	TCATTTCCAACCCCAAGCAT	50.0	45.0	58.1	57.7	1080-1312 bp	0.909091	85.1