Hotspot

petN-trnC-GCA

Genome · 31781-32736 · 956 bp

Back to Astragalus

Pi 0.0535

MarkerSeek score 83.30

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 956
variable_sites: 455
pi: 0.0535
unique_haplotype_count: 57
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	CGATATGGCCGAGTGGTAAG	45.0	55.0	58.0	57.9	681-1082 bp	0.969697	90.2
petN-trnC-GCA_p2	CAGCCCAAGCAAGACTTACT	CGATATGGCCGAGTGGTAAG	50.0	55.0	57.8	57.9	675-1076 bp	0.969697	88.7
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	80-1042 bp	0.909091	87.7
petN-trnC-GCA_p4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	80-1036 bp	0.909091	86.2
petN-trnC-GCA_p5	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	687-1060 bp	0.909091	85.5