Hotspot

psbD-trnT-GGU

Genome · 28027-29092 · 1066 bp

Back to Astragalus

Pi 0.0506

MarkerSeek score 84.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1066
variable_sites: 597
pi: 0.0506
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CACAAAAACGAAACGGTCCC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	827-1227 bp	0.969697	87.2
psbD-trnT-GGU_p2	CCACAAAAACGAAACGGTCC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	828-1228 bp	0.969697	87.2
psbD-trnT-GGU_p3	CCCACAAAAACGAAACGGTC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	829-1229 bp	0.969697	87.2
psbD-trnT-GGU_p4	ACAAAAACGAAACGGTCCCT	GGCGTAAGTCATCGGTTCAA	45.0	50.0	58.2	58.3	826-1226 bp	0.969697	86.6
psbD-trnT-GGU_p5	AACCCACAAAAACGAAACGG	GGCGTAAGTCATCGGTTCAA	45.0	50.0	57.7	58.3	831-1231 bp	0.969697	86.3