Hotspot

trnL-UAA-trnT-UGU

Genome · 13871-14914 · 1044 bp

Back to Astragalus

Pi 0.0645

MarkerSeek score 81.70

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: Genome
length_bp: 1044
variable_sites: 558
pi: 0.0645
unique_haplotype_count: 63
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TGAGCTATCCCGACCATTCT	CGAAGCCATACCCAATCGAA	50.0	50.0	57.9	58.1	2082-2527 bp	0.969697	89.7
trnL-UAA-trnT-UGU_p2	TGAGCTATCCCGACCATTCT	TTGACCCTTTCGCTTTTCCA	50.0	45.0	57.9	57.9	2011-2456 bp	0.969697	89.6
trnL-UAA-trnT-UGU_p3	TGAGCTATCCCGACCATTCT	AGGTCCTCTGTAACGTGACA	50.0	50.0	57.9	58.0	1797-2242 bp	0.924242	88.2
trnL-UAA-trnT-UGU_p4	GCTTCCATTGAGTCTCTGCA	AGGTCCTCTGTAACGTGACA	50.0	50.0	57.9	58.0	1320-1709 bp	0.924242	88.1
trnL-UAA-trnT-UGU_p5	CTCAGGATTGCCCATTGTGA	AGGTCCTCTGTAACGTGACA	50.0	50.0	57.9	58.0	1265-1620 bp	0.893939	86.6