Hotspot

matK-trnK-UUU

Genome · 3554-4268 · 715 bp

Back to Astragalus

Pi 0.0339

MarkerSeek score 83.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: Genome
length_bp: 715
variable_sites: 323
pi: 0.0339
unique_haplotype_count: 50
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGTCCTGTTGGCGAGATCTA	TCGATTCGCCTTCACGATTT	50.0	45.0	57.9	58.0	853-894 bp	0.909091	86.5
matK-trnK-UUU_p2	GAAGTCCTGTTGGCGAGATC	TCGATTCGCCTTCACGATTT	55.0	45.0	58.4	58.0	855-896 bp	0.924242	85.5
matK-trnK-UUU_p3	AGTCCTGTTGGCGAGATCTA	ATCGATTCGCCTTCACGATT	50.0	45.0	57.9	57.8	854-895 bp	0.893939	84.1
matK-trnK-UUU_p4	GAAGTCCTGTTGGCGAGATC	ATCGATTCGCCTTCACGATT	55.0	45.0	58.4	57.8	856-897 bp	0.909091	83.1
matK-trnK-UUU_p5	AGTCCTGTTGGCGAGATCTA	AATCGATTCGCCTTCACGAT	50.0	45.0	57.9	57.8	855-896 bp	0.878788	82.7