Hotspot

rpl16

LSC · 84302-85752 · 1451 bp

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Pi 0.0103

MarkerSeek score 54.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1451
variable_sites: 33
pi: 0.0103
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATTAATTCTCGGGCCC	TCCGCTACAAACCATTCGAG	50.0	50.0	57.9	58.0	1880-1913 bp	1.0	83.0
rpl16_p2	ATCGGCGATTACTAGCTCCT	TCCGCTACAAACCATTCGAG	50.0	50.0	58.1	58.0	1911-1944 bp	1.0	82.7
rpl16_p3	ACACATTAATTCTCGGGCCC	ATATCCTCTGGTGTCGAGGG	50.0	55.0	57.9	58.0	2275-2308 bp	1.0	82.6
rpl16_p4	ACACATTAATTCTCGGGCCC	CTCTGGTGTCGAGGGAATTG	50.0	55.0	57.9	58.0	2270-2303 bp	1.0	82.5
rpl16_p5	ATCGGCGATTACTAGCTCCT	ATATCCTCTGGTGTCGAGGG	50.0	55.0	58.1	58.0	2306-2339 bp	1.0	82.2