Hotspot

rps15-ycf1

SSC · 126514-126935 · 422 bp

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Pi 0.0213

MarkerSeek score 50.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 422
variable_sites: 22
pi: 0.0213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	TGGCAGTCGTTTCAGTATGG	45.0	50.0	58.1	57.9	915-927 bp	1.0	81.5
rps15-ycf1_p2	AACACCATGCATTGATGGGT	GGCAGTCGTTTCAGTATGGT	45.0	50.0	58.1	57.9	914-926 bp	1.0	81.5
rps15-ycf1_p3	AACACCATGCATTGATGGGT	ATGCTTTGCTCGTTCCTGAA	45.0	45.0	58.1	58.1	1189-1201 bp	1.0	81.1
rps15-ycf1_p4	AACACCATGCATTGATGGGT	TGCAATGGGAAGAACGTCAA	45.0	45.0	58.1	58.0	1076-1082 bp	0.8	71.8
rps15-ycf1_p5	AACACCATGCATTGATGGGT	TTGCAATGGGAAGAACGTCA	45.0	45.0	58.1	58.0	1077-1083 bp	0.8	71.8