Hotspot

ndhF-rpl32

SSC · 115425-116551 · 1127 bp

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Pi 0.0117
MarkerSeek score 59.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1127
variable_sites
32
pi
0.0117
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TATTTTTGGTTGCCGTCGGA TCCAATAATTTTTACGAATACGCT 45.0 29.2 58.1 55.3 1294-1320 bp 1.0 42.9
ndhF-rpl32_p2 AAAAAGGAGAAGCCCCACTC TCCAATAATTTTTACGAATACGCT 50.0 29.2 57.7 55.3 1275-1301 bp 1.0 42.9
ndhF-rpl32_p3 GAAAAAGGAGAAGCCCCACT TCCAATAATTTTTACGAATACGCT 50.0 29.2 57.7 55.3 1276-1302 bp 1.0 42.9
ndhF-rpl32_p4 TATTTTTGGTTGCCGTCGGA TCCAATAATTTTTACGAATACGCTT 45.0 28.0 58.1 56.0 1294-1320 bp 1.0 42.9
ndhF-rpl32_p5 TATTTTTGGTTGCCGTCGGA TTCCAATAATTTTTACGAATACGCT 45.0 28.0 58.1 56.0 1295-1321 bp 1.0 42.9