Hotspot

petA-psbJ

LSC · 66161-66970 · 810 bp

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Pi 0.0135

MarkerSeek score 50.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 25
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1242-1313 bp	1.0	85.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1335-1406 bp	1.0	85.0
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1333-1404 bp	1.0	85.0
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1475-1546 bp	1.0	84.9
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1564-1635 bp	1.0	84.7