Hotspot

ndhC-trnV-UAC

LSC · 53099-54215 · 1117 bp

Back to Astilbe

Pi 0.00707
MarkerSeek score 61.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1117
variable_sites
18
pi
0.00707
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCGGAAATTAGAAATGCCA AGAAGGTCTACGGTTCGAGT 40.0 50.0 55.0 57.8 1163-1246 bp 1.0 59.7
ndhC-trnV-UAC_p2 TCCGGAAATTAGAAATGCCAA AGAAGGTCTACGGTTCGAGT 38.1 50.0 55.8 57.8 1163-1246 bp 1.0 58.3
ndhC-trnV-UAC_p3 TCCGGAAATTAGAAATGCCA GAAGGTCTACGGTTCGAGTC 40.0 55.0 55.0 57.5 1162-1245 bp 1.0 56.9
ndhC-trnV-UAC_p4 TCCGGAAATTAGAAATGCCAAA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.6 57.8 1163-1246 bp 1.0 56.3
ndhC-trnV-UAC_p5 TCCGGAAATTAGAAATGCCA GTTCGAGTCCGTATAGCCCT 40.0 55.0 55.0 58.7 1151-1234 bp 1.0 55.8