Hotspot

petN-psbM

LSC · 29717-30366 · 650 bp

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Pi 0.0109

MarkerSeek score 57.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 14
pi: 0.0109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	798-812 bp	1.0	70.6
petN-psbM_p2	GGGGACACAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	820-834 bp	1.0	70.0
petN-psbM_p3	AGGGGACACAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	821-835 bp	1.0	70.0
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	737-751 bp	1.0	69.3
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	798-812 bp	1.0	66.4