Hotspot

trnK-UUU-rps16

LSC · 4306-5163 · 858 bp

Back to Astilbe

Pi 0.011

MarkerSeek score 54.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 858
variable_sites: 22
pi: 0.011
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	AAGGCAGCGGTTTTTAAGGA	55.0	45.0	58.0	58.0	1036-1046 bp	1.0	82.8
trnK-UUU-rps16_p2	CCCTTTCAGGATCAGTCGTG	AGGCAGCGGTTTTTAAGGAA	55.0	45.0	58.0	58.0	1035-1045 bp	1.0	82.8
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	AAGGCAGCGGTTTTTAAGGA	55.0	45.0	58.0	58.0	1035-1045 bp	1.0	82.8
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	AGGCAGCGGTTTTTAAGGAA	55.0	45.0	58.0	58.0	1034-1044 bp	1.0	82.8
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1079-1089 bp	1.0	82.6