Hotspot

rps15

SSC · 126632-126859 · 228 bp

Back to Asteropyrum

Pi 0.00897

MarkerSeek score 44.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 228
variable_sites: 2
pi: 0.00897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	ACAATGCAAGGAACTGTGGT	45.0	45.0	58.1	57.9	1103-1111 bp	1.0	82.4
rps15_p2	AGTCTGCGCACCAATATCTG	ACAATGCAAGGAACTGTGGT	50.0	45.0	58.1	57.9	1415-1423 bp	1.0	81.7
rps15_p3	GAGTCGAAGAACACCATGCA	ACAATGCAAGGAACTGTGGT	50.0	45.0	58.2	57.9	1112-1120 bp	1.0	81.2
rps15_p4	AGCCATAAGGTAGGTCAGCA	ACAATGCAAGGAACTGTGGT	50.0	45.0	58.1	57.9	1539-1547 bp	1.0	81.0
rps15_p5	GCTCGCATCCAATCCAATTG	ACAATGCAAGGAACTGTGGT	50.0	45.0	57.9	57.9	854-856 bp	1.0	80.4