Hotspot

petD

LSC · 80824-82005 · 1182 bp

Back to Asteropyrum

Pi 0.0119
MarkerSeek score 55.50
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1182
variable_sites
14
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 ATTTCCCTTCAACTCCGCAA TCCCTAGATAGATACGTTGTGGT 45.0 43.5 57.7 57.9 1288-1333 bp 1.0 54.5
petD_p2 TTTCCCTTCAACTCCGCAAT TCCCTAGATAGATACGTTGTGGT 45.0 43.5 57.7 57.9 1287-1332 bp 1.0 54.5
petD_p3 ATTTCCCTTCAACTCCGCAA CCCTAGATAGATACGTTGTGGT 45.0 45.5 57.7 56.5 1287-1332 bp 1.0 51.2
petD_p4 TTTCCCTTCAACTCCGCAAT CCCTAGATAGATACGTTGTGGT 45.0 45.5 57.7 56.5 1286-1331 bp 1.0 51.2
petD_p5 TTTCCCTTCAACTCCGCAAT TCCCTAGATAGATACGTTGTGG 45.0 45.5 57.7 56.3 1287-1332 bp 1.0 49.1