Hotspot

ndhD-psaC

SSC · 120433-120559 · 127 bp

Back to Asteropyrum

Pi 0.0248

MarkerSeek score 71.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 127
variable_sites: 3
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCCGCGAATATAGGCAAAA	AAAGGTGTGAATCCGCTTGT	45.0	45.0	57.7	58.0	269-273 bp	1.0	79.8
ndhD-psaC_p2	AGTACCCGCGAATATAGGCA	AAAGGTGTGAATCCGCTTGT	50.0	45.0	58.4	58.0	272-276 bp	1.0	79.5
ndhD-psaC_p3	GTACCCGCGAATATAGGCAA	AAAGGTGTGAATCCGCTTGT	50.0	45.0	57.6	58.0	271-275 bp	1.0	78.9
ndhD-psaC_p4	ACCCGCGAATATAGGCAAAA	GGATTTCTTGAGTGTCCGGG	45.0	55.0	57.7	58.3	244-248 bp	1.0	78.2
ndhD-psaC_p5	AGTACCCGCGAATATAGGCA	GGATTTCTTGAGTGTCCGGG	50.0	55.0	58.4	58.3	247-251 bp	1.0	77.8