Hotspot

petA-psbJ

LSC · 67661-68113 · 453 bp

Back to Asteropyrum

Pi 0.0163

MarkerSeek score 69.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 7
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCATCTGTTATTCTGGCA	ATTCTGGGTTGGGTTCATCC	45.0	50.0	57.2	57.5	565-605 bp	1.0	73.4
petA-psbJ_p2	TTGGCATCTGTTATTCTGGCA	ATTCTGGGTTGGGTTCATCC	42.9	50.0	57.9	57.5	566-606 bp	1.0	71.2
petA-psbJ_p3	GGCATCTGTTATTCTGGCAC	ATTCTGGGTTGGGTTCATCC	50.0	50.0	56.9	57.5	564-604 bp	1.0	70.7
petA-psbJ_p4	CGCGTGTCCAAGGTCTTTTT	ATTCTGGGTTGGGTTCATCC	50.0	50.0	59.3	57.5	592-632 bp	1.0	69.0
petA-psbJ_p5	TGGCATCTGTTATTCTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.2	58.1	614-654 bp	1.0	68.6