Hotspot

ndhC-trnV-UAC

LSC · 54876-56113 · 1238 bp

Back to Asteropyrum

Pi 0.0265

MarkerSeek score 64.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 31
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTGTGCCCGCTGTTAAAATG	TGGTTGGTCCAATTGAGGTG	50.0	50.0	58.0	58.0	2407-2441 bp	1.0	83.5
ndhC-trnV-UAC_p2	CAATGCTCCTTTTCGCCATG	TGGTTGGTCCAATTGAGGTG	50.0	50.0	58.1	58.0	2042-2085 bp	1.0	83.5
ndhC-trnV-UAC_p3	CTGTGCCCGCTGTTAAAATG	AAGAACGCAAGGGCTATAGC	50.0	50.0	58.0	58.0	2575-2613 bp	1.0	83.1
ndhC-trnV-UAC_p4	AGAGAAGCGGCCATAAACTG	TGGTTGGTCCAATTGAGGTG	50.0	50.0	58.0	58.0	2315 bp	0.5	59.7
ndhC-trnV-UAC_p5	AGAGAAGCGGCCATAAACTG	AAGAACGCAAGGGCTATAGC	50.0	50.0	58.0	58.0	2487 bp	0.5	59.4