Hotspot

trnF-GAA-ndhJ

LSC · 52385-53123 · 739 bp

Back to Asteropyrum

Pi 0.0145

MarkerSeek score 69.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 10
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	50.0	45.0	58.1	57.8	825-876 bp	1.0	83.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	797-848 bp	1.0	82.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	50.0	50.0	58.1	58.3	827-878 bp	1.0	81.7
trnF-GAA-ndhJ_p4	GGAATGATGCATCGGGAAGA	TCCACGCCTGAAACGTATTT	50.0	45.0	57.7	57.8	884-935 bp	1.0	81.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATT	50.0	45.0	58.1	57.6	826-877 bp	1.0	81.4