Hotspot

trnT-GGU-psbD

LSC · 33903-35710 · 1808 bp

Back to Asteropyrum

Pi 0.0219
MarkerSeek score 60.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1808
variable_sites
39
pi
0.0219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA TCTACCAAGGGCTATAGTCA 50.0 45.0 57.9 54.3 1901-1950 bp 1.0 52.5
trnT-GGU-psbD_p2 GGCGTAAGTCATCGGTTCAA TCTACCAAGGGCTATAGTCA 50.0 45.0 58.3 54.3 1864-1913 bp 1.0 51.1
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA ATCTACCAAGGGCTATAGTCA 50.0 42.9 57.9 55.0 1902-1951 bp 1.0 49.6
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA TCTACCAAGGGCTATAGTCAT 50.0 42.9 57.9 55.0 1901-1950 bp 1.0 49.6
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA GGTAAATCTACCAAGGGCTA 50.0 45.0 57.9 53.8 1907-1956 bp 1.0 48.4