Hotspot

petN-psbM

LSC · 30132-31469 · 1338 bp

Back to Asteropyrum

Pi 0.0161

MarkerSeek score 63.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1338
variable_sites: 21
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1487-1493 bp	1.0	71.8
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1493-1499 bp	1.0	70.3
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	1487-1493 bp	1.0	67.6
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	57.8	56.1	1493-1499 bp	1.0	66.1
petN-psbM_p5	TTGCTTGGGCTGCTTTAATG	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.3	57.6	1485-1491 bp	1.0	65.9