Hotspot

trnR-UCU-atpA

LSC · 10848-10978 · 131 bp

Back to Asteropyrum

Pi 0.0465

MarkerSeek score 80.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 6
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGAATGAAAAGCGTCCATTGT	AAGACACTCACCGAAGAAGC	42.9	50.0	57.4	57.8	298-300 bp	1.0	68.3
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	CGAAGAAGCGGAAGTCCTTT	42.9	50.0	57.4	58.2	287-289 bp	1.0	68.0
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AAGACACTCACCGAAGAAGC	47.6	50.0	57.3	57.8	238-240 bp	1.0	67.9
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	CGAAGAAGCGGAAGTCCTTT	47.6	50.0	57.3	58.2	227-229 bp	1.0	67.6
trnR-UCU-atpA_p5	AGTAGGAATGAAAAGCGTCCA	AAGACACTCACCGAAGAAGC	42.9	50.0	57.3	57.8	302-304 bp	1.0	67.3