Hotspot

petD

LSC · 77080-78323 · 1244 bp

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Pi 0.0125
MarkerSeek score 60.20
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1244
variable_sites
50
pi
0.0125
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 ATTTCCCTTGAACTCCGCAA TGAAGCGACTGTTCCCTAGA 45.0 50.0 57.7 58.1 1361-1437 bp 1.0 82.4
petD_p2 TTTCCCTTGAACTCCGCAAT TGAAGCGACTGTTCCCTAGA 45.0 50.0 57.7 58.1 1360-1436 bp 1.0 82.4
petD_p3 ATTTCCCTTGAACTCCGCAA CTTTGAAGCGACTGTTCCCT 45.0 50.0 57.7 58.1 1364-1440 bp 1.0 82.1
petD_p4 TTTCCCTTGAACTCCGCAAT CTTTGAAGCGACTGTTCCCT 45.0 50.0 57.7 58.1 1363-1439 bp 1.0 82.1
petD_p5 TTTCCCTTGAACTCCGCAAT ACTTTGAAGCGACTGTTCCC 45.0 50.0 57.7 58.4 1364-1440 bp 1.0 79.8