Hotspot

ndhA

SSC · 119468-121640 · 2173 bp

Pi 0.00868

MarkerSeek score 61.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2173
variable_sites: 65
pi: 0.00868
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	CCGCCGGGGTTTATTAATTTG	40.0	47.6	55.2	57.9	2292-2327 bp	1.0	52.9
ndhA_p2	TTGTTTGAGACAAGTCGTGA	CCGCCGGGGTTTATTAATTTG	40.0	47.6	55.2	57.9	2293-2328 bp	1.0	52.9
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	CCGCCGGGGTTTATTAATTTG	38.1	47.6	56.0	57.9	2293-2328 bp	1.0	51.3
ndhA_p4	ACCATAGGGAACATATCGTGA	CCGCCGGGGTTTATTAATTTG	42.9	47.6	55.9	57.9	2349-2384 bp	1.0	50.8
ndhA_p5	TGGCCATAATTCATGAACTCAGT	CCGCCGGGGTTTATTAATTTG	39.1	47.6	58.1	57.9	2373-2408 bp	1.0	50.1