Hotspot

rpl32-trnL_uag

SSC · 113316-114122 · 807 bp

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Pi 0.015
MarkerSeek score 65.60
Primer available yes
Label rpl32-trnL_uag

Feature Metadata

label_name
rpl32-trnL_uag
feature_type
igs
region
SSC
length_bp
807
variable_sites
48
pi
0.015
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_uag_p1 GGGCGTCGTTAAAAGCTTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 899-968 bp 1.0 85.5
rpl32-trnL_uag_p2 GGGCGTCGTTAAAAGCTTTG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 900-969 bp 1.0 85.5
rpl32-trnL_uag_p3 GGCGTCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 898-967 bp 1.0 84.9
rpl32-trnL_uag_p4 GGCGTCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 899-968 bp 1.0 84.9
rpl32-trnL_uag_p5 GGGCGTCGTTAAAAGCTTTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.3 57.9 909-978 bp 1.0 84.6